Extended Data Fig. 3. VH locus CBEs are more dense and more potent than Vκ locus CBEs. Related to Fig. 2.
a, Locations of CBEs in the VH locus (left) and Vκ locus (right). There are 119 annotated CBEs in the 2.84 Mb VH locus and 55 annotated CBEs in the 3.16 Mb Vκ locus. The two loci are shown on the same genomic scale to reflect the difference in CBE density. In the Vκ locus, rightward CBEs are shown in red, leftward CBEs are in blue. In the VH locus, leftward CBEs are shown in red, rightward CBEs are in blue. b, Average enrichment of CTCF ChIP-seq signal within ± 1 kb region across all annotated CBEs in the VH locus (left) and Vκ locus (right) in RAG-deficient parental v-Abl cells (blue) or CTCF-depleted v-Abl cells (red). Data are presented as average signal counts (solid blue or red line) ± s.e.m. (blue or red shade) from 3 biological repeats. The CTCF ChIP-seq data shown were extracted from data deposited in the context of a prior study of RAG-deficient parental and CTCF-depleted v-Abl cells6.