Extended Data Fig. 4. Igh-Igκ hybrid line generated by targeted chromosomal translocation maintains normal D-to-JH and Vκ-to-Jκ rearrangements. Related to Fig. 4.
a, Confirmation of translocation junction in Igh-Igκ hybrid v-Abl line (shown in Fig. 4a) by PCR/Sanger sequencing. The sgRNA sequences are underlined, sgRNA cut sites are indicated by red arrows, and the Cas9 PAM sequences are labeled in red. b, Whole chromosome painting results with probes tiling chromosome 6 (green) and chromosome 12 (red) in single Jκ5-single Igh v-Abl cells (“Parental”, left) and Igh-Igκ hybrid v-Abl cells (“Translocation”, right). After translocation, a chr12-chr6 fusion chromosome is detected with half of chr6 appended onto chr12. The reciprocal translocation also placed the small telomeric portion (~7 Mb) of chr12 onto chr6, which is below the detectable size of painting experiments. c, Absolute level of individual Vκ-to-Jκ joins in Igh-Igκ hybrid line with Jκ5 bait. d, Absolute level of individual D-to-JH joining in Igh-Igκ hybrid line with JH1-4 bait. Vκ and D usage data are presented as mean ± s.e.m. from 3 biological repeats.