Fig. 7.

Irf8 deletion impairs the proliferation and antigen uptake of kidney DCs during AKI. (A) Experimental design. Twelve hours after IR surgery, EdU was injected i.p. 12 h before the mice were sacrificed. (B) Histogram plots showing EdU incorporation in kidney MPCs from Irf8^+/+ (black) or Irf8^−/− (red) mice 1 day after AKI and from healthy Irf8^+/+ mice (gray). Representative images of three independent experiments are shown. (C) Percentages of EdU-positive cells in each MPC subset (n = 3–5 mice per group). (D-E) Flow cytometry analysis of antigen uptake by kidney MPCs. Kidneys were harvested from AKI mice and healthy mice. MPCs freshly isolated from kidneys were exposed to ovalbumin-Alexa Fluor 488 (OVA-AF488). Antigen incorporation was visualized by calculating the mean fluorescence intensity (MFI) of OVA-AF488 in each subset (n > 3 mice per group). The data are shown as the mean ± SD. *p < 0.05, ***p < 0.001, and ****p < 0.0001. One-way ANOVA. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)