Figure 2.

Activation of dLS^GLP−1Rneurons has no significant effect on food intake.

(A) Brain schematic of viral injection for dLS^GLP−1R neuron activation.

(B) Transduction of dLS^GLP−1R neurons with hM3Dq-mCherry.

(C) CNO application has no significant effect on the resting membrane potential and the firing rate of dLS^GLP−1R neurons (paired t-test, t(17), p = 0.2523, n = 17 cells from 3 mice), after CNO application, 35% of neurons exhibited resting membrane potential hyperpolarization, and 29% of neurons exhibited resting membrane potential depolarization.

(D–F) Chemogenetic activation of dLS^GLP−1R neurons has no significant effect on food intake (D) during the dark cycle when fed (two-way ANOVA, no main effect of Group: F(1,105) = 3.402, p = 0.0679; main effect of Time: F(4,105) = 36.10, p < 0.0001, no interaction between Group and Time: F(4,105) = 0.3067, p = 0.8730.), (E) during the light cycle when fed (two-way ANOVA, no main effect of Group: F(1,105) = 0.06226, p = 0.8034; main effect of Time: F(4,105) = 18.67, p < 0.0001, no interaction between Group and Time: F(4,105) = 0.01724, p = 0.9994), and (F) refeeding following an overnight fast (two-way ANOVA, no main effect of Group: F(1,105) = 0.1432, p = 0.7059; main effect of Time: F(4,105) = 84.5, p < 0.0001, no interaction between Group and Time: F (4,105) = 0.04575, p = 0.9960). n = 13 control and 10 hM3Dq mice.

(G–I) Local inhibitory connections in dLS. Schematic showing the experiment used to record postsynaptic currents in local dLS neurons induced by optogenetic stimulation of dLS^GLP−1R neurons (G). In brain slice recordings, blue light stimulation evoked robust IPSCs, which were blocked by picrotoxin but not CNQX (H–I) (one-way ANOVA, F (4,25) = 98.81, p < 0.0001, ∗∗∗∗Sidak's multiple comparisons test p < 0.0001 vs. ACSF). n = 6 cells from 3 mice.

Data are presented as mean ± SEM.