Figure 5.

Activation of dLS^GLP−1R projection neurons has no significant effect on food intake.

(A) Brain schematic of viral injection for dLS^GLP−1R → LHA neuron activation.

(B) Transduction of dLS^GLP−1R → LHA neurons with hM3Dq-mCherry.

(C) CNO application depolarized the resting membrane potential and increased the firing rate of dLS^GLP−1R→LHA projection neurons (paired t-test, t(8) = 2.702, p = 0.0306, n = 8 cells from 3 mice).

(D–F) Chemogenetic activation of dLS^GLP−1R → LHA projection neurons has no significant effect on food intake (D) during the dark cycle when fed (two-way ANOVA, no main effect of Group: F(1,45) = 0.07858, p = 0.7805; main effect of Time: F(4,45) = 23.01, p < 0.0001, no interaction between Group and Time: F(4,45) = 0.1826, p = 0.9463), (E) during the light cycle when fed (two-way ANOVA, a marginally significant main effect of Group: F(1,50) = 3.272, p = 0.0765; main effect of Time: F(4,50) = 6.024, p = 0.0005, no interaction between Group and Time: F(4,50) = 0.4554, p = 0.7680). (F) Chemogenetic activation suppressed food intake during refeeding following an overnight fast (two-way ANOVA, main effect of Group: F(1,50) = 11.01, p = 0.0017; main effect of Time: F(4,50) = 64.4, p < 0.0001, no interaction between Group and Time: F(4,50) = 1.013, p = 0.4096). n = 7 control and 5 hM3Dq mice.

Data are presented as mean ± SEM.