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. 2024 May 17;85:101960. doi: 10.1016/j.molmet.2024.101960

Figure 6.

Figure 6

dLSGLP−1R→LHA activation suppresses feeding.

(A and B) ChR2-EYFP expression in the dLS (A) and axonal projections in the LHA (B) in Glp1r-ires-Cre mice.

(C) Photostimulation of dLSGLP−1R → LHA projections significantly suppressed food intake (two-way ANOVA, main effect of Group: F(1,36) = 3.382, p = 0.0742; main effect of Stimulation: F(2,36) = 3.816, p = 0.0314, no interaction between Group and Stimulation: F(2,36) = 2.576, p = 0.0900. ∗Sidak's multiple comparisons test p < 0.05). Data are presented as mean + SEM. n = 6 controls and 8 ChR2 mice.

(D) Experimental paradigm for recording postsynaptic currents in LHA slices induced by optogenetic stimulation of dLSGLP−1R neurons.

(E) Representative evoked IPSCs after paired-pulse optogenetic stimulation.

(F) Quantification of evoked IPSCs (paired t-test, t(10) = 2.312, p = 0.0461) and PPR (paired t-test, t(10) = 3.135, p = 0.0120) before and after Exn4 application. n = 10 cells from 3 mice. ∗p < 0.05.