Table 2.

Central composite experimental design using factors having positive effects on lyase SG4 + production

Runs	Yeast extract (%, w/v)	Sodium alginate (%, w/v)	Agitation (rpm)	Inoculum volume (%, v/v)	Response (IU)
1	1.38	0.25	200	10	0.461
2	0.625	0.25	200	10	0.837
3	0.25	0.40	100	15	0.303
4	0.25	0.40	300	5	0.297
5	1.00	0.10	100	10	0.251
6	0.625	0.55	200	10	0.476
7	0.625	0.25	400	10	0.353
8	0.625	0.25	200	20	0.6268
9	0.25	0.10	300	5	0.314
10	0.0	0.25	200	10	0.464
11	0.625	0.25	200	10	0.839
12	0.625	0.25	200	10	0.830
13	1.0	0.10	100	15	0.248
14	0.625	0.25	0.00	10	0.1764
15	0.25	0.40	100	5	0.303
16	1.00	0.10	300	5	0.435
17	0.25	0.10	300	15	0.301
18	0.625	0.0	200	10	0.0934
19	0.625	0.25	200	10	0.846
20	0.625	0.25	200	10	0.842
21	1.00	0.40	100	5	0.294
22	0.25	0.4	300	15	0.601
23	0.25	0.10	100	15	0.188
24	0.625	0.25	200	00	0
25	0.25	0.10	100	5	0.217
26	1.0	0.40	300	5	0.553
27	0.625	0.25	200	10	0.849
28	1.00	0.40	100	15	0.294
29	1.00	0.40	300	15	0.648
30	1.00	0.10	300	15	0.339