Figure 3.

The effect and mechanism of miR-146a on chondrocyte apoptosis in vitro. (A, B) The apoptosis rate of chondrocytes was determined using FCM. Detection was performed 24 h after transfection with the miR-146a mimic or inhibitor. (C) Relative mRNA expression levels of TLR4, TRAF6, and NF-κB were detected using qPCR. (D, E) The protein levels of TLR4, TRAF6, p-NF-κB, and NF-κB were detected using WB. Data are expressed as the mean ± SEM (n = 3). **** p < 0.00001, *** p < 0.0001, ** p < 0.01, * p < 0.05.