Figure 4.

Extraction and identity assessment of exosome-like vesicles from FLSs pretreated with the miR-146a mimic. (A) FLSs were isolated and cultured in cell medium then transfected with the miR-146a mimic and miR-146a mimic NC. Exosome-like vesicles were extracted from the supernatant using supercentrifugation. (B) The morphology of exosome-like vesicles was observed using TEM (×100000). (C) The exosome particle size was measured using NTA (nm). (D) WB detected the exosome characteristic markers, CD9, TSG101, and HSP70. (E) qPCR was performed to detect the expression levels of miR-146a in exosome samples from the cell supernatant. Data are expressed as the mean ± SEM (n = 3). *** p < 0.0001.