FIG. 6.

L-deficient FMDV replicon induces specific eIF4A and eIF4G cleavage. (A) The structure of the FMDV replicon (pT7Rep) is indicated together with the parental infectious cDNA pT7S3 (18) and the sequences removed (by digestion of the cDNA with ApaI and religation) to generate the nonreplicating derivatives of the replicon (pT7RepΔApa) and its parent (pT7S3ΔApa). Restriction sites: A, ApaI; H, HpaI; RV, EcoRV. (B and C) RNA transcripts prepared from HpaI-linearized plasmids by using T7 RNA polymerase were electroporated into BHK cells. After 6 h cell extracts were prepared and analyzed for eIF4G (B) and eIF4A (C) as for Fig. 3 and 4.