Fig. 1.

Cell viability in fibroblasts exposed to UVA irradiation and after pre- and post-treatment with different concentrations of punicalagin. (A) Cell viability was expressed as % of the control (unexposed and untreated cells). Punicalagin was used at different concentrations (0, 5, 10 and 25 μM). Statistical analysis was performed by comparing data under the same experimental conditions (0, 1 and 2 h UVA exposure). Note the cytoprotective effect of 10 μM punicalagin after 1 and 2 h of UVA exposure. **p < 0.01 (between untreated cells, 0 μM); #p < 0.05 (for 1 h UVA exposure); ° p < 0.05 (for 2 h UVA exposure) by one-way ANOVA (with Geisser-Greenhouse's correction) (B) Post-treatment with 10 μM punicalagin did not increase cell viability after UVA exposure (both at 1 and 2 h irradiation). **p < 0.01 (for untreated 0 μM and 10 μM post-treated cells) with t-test.