FIG. 9.

Metal content and enzymatic activity of EDTA- and zinc-treated GST-2C. Bacterially expressed GST-2C was bound to glutathione-Sepharose beads in the presence of 1 mM EDTA or 0.1 mM zinc acetate. The beads were washed four times with 100 bead volumes each and eluted. (A) Metal content of the differently treated GST-2C preparations. (B) Specific ATPase activity of the two preparations. Inorganic phosphate released from ATP was determined by a colorimetric assay (44). The average and standard deviation of triplicate measurements are shown.