Table 2.
Sequencing results.
| ID | Pre-digestion | Digestion time | Sequenced reads | Human aligned and filtered | Human depth (x) | Human Damage (3') | Animal aligned and filtered | Animal depth (x) | Damage animal (3') | Human:animal reads | Proportion of target animal reads in run | Species assessment | Overall assessment |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Dz15136 | 2.5 | NO | 7596370 | 30117 | 144.77 | 0 | 556 | 2.67 | 0.46 | 54.17 | 0.00007 | Bos taurus | Possible species ID |
| Dz2724 | 2.5 | NO | 3985647 | 955 | 3.93 | 0.02 | 21 | 0.07 | 0.02 | 45.48 | 0.00001 | Bos taurus | Fail |
| Dz19364 | 2.5 | NO | 3486590 | 904 | 4.00 | 0 | 60 | 0.20 | 0.02 | 15.07 | 0.00002 | Cervus elaphus | Possible species ID |
| Dz19352 | 2.5 | NO | 4894516 | 1190 | 5.00 | 0 | 164 | 0.1 | 0.08 | 7.26 | 0.00003 | Alces alces | Possible species ID |
| Dz15129 | 2.5 | NO | 66595 | 1 | – | 0.01 | – | – | 0.01 | – | – | - | Fail |
| Dz19285 | 2.5 | NO | 3440798 | 10240 | 46.73 | 0.01 | 164 | 0.87 | 0.01 | 62.44 | 0.00005 | Sus scrofa | Fail: Implausible species ID; must be animal glue contaminant |
| ML4529 | 2.5 | NO | 3594736 | 4592 | 18.14 | 0.02 | 181 | 0.8 | 0.02 | 25.37 | 0.00005 | Sus scrofa | Fail: Implausible species ID; must be animal glue contaminant |
| ML4530 | 2.5 | NO | 5974246 | 741 | 2.76 | 0.01 | 58 | 0.17 | 0.02 | 12.78 | 0.00001 | Bos taurus | Fail: Implausible species ID; must be animal glue contaminant |
| ML4533 | 2.5 | NO | 5223363 | 4454 | 16.62 | 0.02 | 139 | 0.47 | 0.03 | 32.04 | 0.00003 | Bos taurus | Fail: Implausible species ID; must be animal glue contaminant |
| ML4534 | 2.5 | NO | 4144995 | 28306 | 134.02 | 0.03 | 892 | 2.97 | 0.03 | 31.73 | 0.00022 | Bos taurus | Fail: Implausible species ID; must be animal glue contaminant |
| ML4532 | 2.5 | NO | 3737677 | 22929 | 94.54 | 0.01 | 181 | 0.69 | 0.01 | 126.68 | 0.00005 | Capra hircus | Fail: Implausible species ID; must be animal glue contaminant |
| Poi1 | 2.0 | NO | 5406771 | 135 | 1.70 | 0 | – | – | 0.01 | – | – | - | Fail |
| Poi2 | 2.0 | NO | 8950423 | 164 | 0.63 | 0.03 | – | – | 0.00 | – | – | - | Fail |
| LQ10 | 2.0 | NO | 4177141 | 135 | 0.57 | 0.03 | – | – | 0.00 | – | – | - | Fail |
| Fe4 | 2.0 | NO | 6870813 | 1843 | 8.55 | 0.01 | – | – | 0.01 | – | – | - | Fail |
| IST4 | 2.0 | NO | 1269927 | – | – | 0 | – | – | 0.10 | – | – | - | Fail |
| St755 | 2.5 | NO | 13668029 | 532 | 2.43 | 0.04 | – | – | 0.00 | – | – | - | Fail |
| St766 | 2.5 | NO | 5702910 | 112 | 0.49 | 0 | – | – | 0.00 | – | – | - | Fail |
| St766b | 2.5 | NO | 4071809 | 138 | 0.69 | 0.03 | 30 | 0.14 | 0.09 | 4.60 | 0.00001 | Bos taurus | Fail: Implausible species ID; must be animal glue contaminant |
| St1017 | 2.5 | NO | 4699441 | 77 | 0.35 | 0 | – | – | 0.20 | – | – | - | Fail |
| St673_powder | 18.0 | NO | 8476281 | 126 | 1.00 | 0.01 | 1501 | 5.4 | 0.40 | 0.08 | 0.00018 | Cervus elaphus | Possible species ID |
| St784 | 2.5 | NO | 8019576 | 877 | 3.50 | 0.01 | – | – | – | – | – | - | Fail |
| St694 | 2.5 | NO | 13780889 | 12443 | 55.3 | 0 | 779 | 2.5 | 0.35 | 15.97 | 0.00006 | Bison bonasus | Possible species ID |
| St801 | 2.5 | NO | 10825776 | 30482 | 172.00 | 0.01 | 2976 | 11 | 0.44 | 10.24 | 0.00027 | Capra hircus | Possible species ID |
| Dz13771 | 2.5 | NO | 6965180 | 2441 | 10.70 | 0.01 | 1398 | 10.78 | 0.42 | 1.75 | 0.00020 | Capra hircus | Possible species ID |
| Dz12076 | 2.5 | NO | 7460673 | 13767 | 65.4 | 0.02 | – | – | – | – | – | - | Fail |
| Dz19307 | 2.5 | NO | 7050983 | 9560 | 42.00 | 0.01 | – | – | – | – | – | - | Fail |
| Samele Klde | 2.5 | NO | 8555243 | 313 | 0.97 | 0 | 83 | 0.26 | – | 3.77 | 0.00001 | Cervus elaphus | Possible species ID |
| CHU1 | 1.5 | YES | 4444816 | 4204 | 0.69 | 0.01 | 3 | – | 0.12 | 1401.33 | 0.00000 | - | Fail |
| CHU2 | 1.5 | YES | 4340206 | 10742 | 0.75 | 0.01 | 2200 | 8.222 | 0.39 | 4.88 | 0.00051 | Cervus elaphus | Possible species ID |
| CHU3 | 1.5 | YES | 5786327 | 30464 | 1.11 | 0.02 | 1876 | 6.65 | 0.25 | 16.24 | 0.00032 | Cervus elaphus | Possible species ID |
| CHU4 | 1.5 | YES | 1367638 | 2497 | 0.49 | 0.04 | 29371 | 176.91 | 0.34 | 0.09 | 0.02148 | Cervus elaphus | Possible species ID |
| KS3; RGM.1333607 | 2.0 | YES | 4854073 | 255 | 14.75 | 0 | 0 | – | – | – | – | - | Fail |
| KS6; RGM.1333610 | 2.0 | YES | 4274500 | 2278 | 6.37 | 0.02 | 1 | – | 0.01 | 2278.00 | 0.00000 | - | Fail |
| NR1 | 2.0 | YES | 5041283 | 1262 | 5.94 | 0.01 | 0 | – | 0.01 | – | – | - | Fail |
| NR2 | 2.0 | YES | 4737747 | 3439 | 2.19 | 0 | 0 | – | 0.01 | – | – | - | Fail |
| StEx1 | 2.0 | YES | 5737159 | 14018 | – | 0 | 33 | – | 0.18 | 424.79 | 0.00001 | - | Fail |
| StEx2 | 2.0 | YES | 4605353 | 557 | – | 0.02 | 17 | – | 0.23 | 32.76 | 0.00000 | - | Fail |
| StEx3 | 2.0 | YES | 5015779 | 1504 | 12.33 | 0 | 1138 | 3.51 | 0.33 | 1.32 | 0.00023 | Bison bonasus | Possible species ID |
| TB1 | 1.5 | YES | 157358 | 184 | 0.00 | 0.11 | 147 | 0.56 | 0.43 | 1.25 | 0.00093 | Cervus elaphus | Possible species ID |
| TB2 | 1.5 | YES | 33254 | 72 | 0.16 | 0 | 13904 | 46.60 | 0.36 | 0.01 | 0.41812 | Cervus elaphus | Possible species ID |
| TB3 | 1.5 | YES | 3886632 | 196 | 1.86 | 0.01 | 9708 | 33.77 | 0.43 | 0.02 | 0.00250 | Cervus elaphus | Possible species ID |
| GI-5866 | 2.0 | NO | 5506646.00 | 773.00 | 3.40 | 0.04 | – | – | 0.51 | – | – | – | Fail |
| GI-5817 | 2.0 | NO | 8376119.00 | 3529.00 | 15.34 | 0 | – | – | 0.01 | – | – | – | Fail |
| GI-7963 | 2.0 | NO | 8015545.00 | 335.00 | 1.12 | 0.01 | 1349 | 4.01 | 0.25 | 0.25 | 0.00 | Cervus elaphus | Possible species ID |
| GI-5835 | 2.0 | NO | 8290699.00 | 18773.00 | 95.23 | 0.01 | – | – | 0.01 | – | – | – | Fail |
| GI-7964 | 2.0 | NO | 2778282.00 | 63.00 | 0.12 | 0.05 | 230 | 0.43 | 0.23 | 0.27 | 0.00 | Cervus elaphus | Possible species ID |
| GI-5869 | 2.0 | NO | 790674.00 | 11.00 | – | 0 | 16 | – | 0.00 | 0.69 | 0.00 | – | Fail |
| GI-7968 | 2.0 | NO | 6211225.00 | 492.00 | 2.20 | 0.02 | 13 | – | 0.08 | 37.85 | 0.00 | – | Fail |
| GI-7969 | 2.0 | NO | 3721428.00 | 13.00 | – | 0 | 13 | – | 0.00 | 1.00 | 0.00 | – | Fail |
| EXTRACTION-BLANK1 | – | – | 196250 | 40 | – | 0 | – | – | 0.40 | – | – | - | Blank |
| LIBRARY-BLANK1 | – | – | 66595 | 1 | – | 0 | – | – | – | – | – | - | Blank |
| EXTRACTION-BLANK2 | – | – | 285807 | 39 | – | 0 | – | – | – | – | – | - | Blank |
| LIBRARY-BLANK2 | – | – | 278834 | 2 | – | 0 | – | – | – | – | – | - | Blank |
| EXTRACTION-BLANK3 | – | – | 941044 | 18 | – | 0 | – | – | – | – | – | - | Blank |
| LIBRARY-BLANK3 | – | – | 620282 | 8 | – | 0 | – | – | – | – | – | - | Blank |
| EXTRACTION-BLANK4 | – | – | 578274 | 45 | 0.23 | 0 | – | – | – | – | – | - | Blank |
| EXTRACTION-BLANK5 | – | – | 52983 | 5 | – | 0 | – | – | – | – | – | - | Blank |
| LIBRARY-BLANK4 | – | – | 307915.00 | 2.00 | – | 0 | – | – | – | – | – | – | Blank |
| EXTRACTION-BLANK6 | – | – | 3238.00 | 69.00 | – | 0 | 69 | – | 0.00 | 1.00 | 0.02 | – | Blank |
| LIBRARY-BLANK5 | – | – | 11907 | 9 | – | 0 | 9 | – | 0 | 1.00 | 0.00076 | - | Blank |