Table 1.
List of oligonucleotides used to analyze the immunometabolic crosstalking on Aedes fluviatilis Wolbachia pipientis symbiosis
| Oligonucleotide name | Oligonucleotide | Gene code |
|---|---|---|
| Rp49 qPCR Fw | GATGCAGAACCGTGTCTACT | MW574127 |
| Rp49 qPCR Rv | AGCTTACTCGTTTTCTTGCG | |
| CASPAR qPCR Fw | GCGGATCGAGCAAAGCAAGA | OM830326 |
| CASPAR qPCR Rv | CAGTCGGTGCCCTTATCCTA | |
| CACTUS qPCR Fw | CCGTAATTTTGGGCACTCAT | OM830327 |
| CACTUS qPCR Rv | CATTAACGCAAGGGAAGGAA | |
| WSP Fw qPCR | ATCTTTTATAGCTGGTGGTGGT | GQ917108 |
| WSP Rv qPCR | GGAGTGATAGGCATATCTTCAAT | |
| WSP Fw (Check) | TGGTCCAATAAGTGATGAAGAAAC | GQ917108 |
| WSP Rv (Check) | AAAAATTAAACGCTACTCCA | |
| Cecropin qPCR Fw | CGATTTGACGTTCGAGATGA | MW574131 |
| Cecropin qPCR Rv | CGTTTGCCTACTCCTTCCAA | |
| Defensin qPCR Fw | AACTCTCCTCTCACGCCGTA | MW574130 |
| Defensin qPCR Rv | TACGAGCGAACATCATCAGC | |
| Gambicin qPCR Fw | AGATGCGCTGGTGTTCGTAT | OM830330 |
| Gambicin qPCR Rv | TCACTGCAGGTTCTGATTGC | |
| Rel1 qPCR Fw | CAGCCAATCAGCAACAGAAA | MW574128 |
| Rel1 qPCR Rv | ATTCGTTTGATGGGCGATAG | |
| Rel2 qPCR Fw | ATTGTTTCCGTCTGGATTCG | MW574129 |
| Rel2 qPCR Rv | TCACGCAGAACGTATGAAGC | |
| GSK3 qPCR Fw | TCATCAAAGTCCTCGGAACG | OM830324 |
| GSK3 qPCR Rv | ATCGCATCTGGTGGAGTACG | |
| GFP t7 Fw | TAATACGACTCACTATAGGGACGTAAACGGCCACAAGTTCAGCGTGTC | EF152770.1 |
| GFP t7 Rv | TAATACGACTCACTATAGGGTCACGAACTCCAGCAGGACCATGTCATC | |
| Rel2 t7 Fw | TAATACGACTCACTATAGGGCATTGCCGAGGAACTGAGCA | MW574129 |
| Rel2 t7 Rv | TAATACGACTCACTATAGGGCCCAATCTTGCCATGCTTCTT | |
| GSK3 t7 Fw | TAATACGACTCACTATAGGGCCCGTTATGGGAGGCATGAA | OM830324 |
| GSK3 t7 Rv | TAATACGACTCACTATAGGGTCGGTTCCCCATGCAGAAGT |
Primer sequences used to evaluate immunometabolism-related gene expression and perform dsRNA synthesis to gene knockdown by RNA interference.