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. 2024 May 8;103(7):103841. doi: 10.1016/j.psj.2024.103841

Figure 4.

Figure 4

MiR-24-5p resists proliferation and promotes apoptosis of GCs. (A, B) After transfection with miR-24-5p inhibitor or mimic, the mRNA levels of CCND1, CCND2, CDK2 and PCNA were assessed by qPCR. n = 9. (C, D) After transfection with miR-24-5p inhibitor or mimic, the growth curve of GCs was evaluated by CCK-8 reagent. n = 8. (E, F) The proliferation status of GCs was determined by EdU assay after transfection of miR-24-5p. n = 3. (G, H) After transfection with miR-24-5p inhibitor or mimic, the mRNA levels of BCL2, Caspase3, Caspase8 and Caspase9 were assessed by qPCR. n = 9. (I, J) The apoptosis state of GCs determined by TUNEL assay after transfection of miR-24-5p 48h. n = 3. (K) After transfection with miR-24-5p inhibitor or mimic, the protein level of β-tubulin, Caspase9 was measured by western blot. n = 3. Values represent mean ± SEM. * P < 0.05 and ⁎⁎P < 0.01.