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. 2024 May 8;103(7):103841. doi: 10.1016/j.psj.2024.103841

Figure 8.

Figure 8

CircRAB11A regulates GC proliferation and apoptosis through ERK1/2 and PI3K/AKT signaling pathways by targeting EGFR. (A) After transfection with EGFR interference vector, the mRNA levels of CCND1, CCND2, CDK2 and PCNA were assessed by qPCR. n = 9. (B) After transfection with EGFR interference vector, the mRNA levels of BCL2, Caspase3, Caspase8 and Caspase9 were measured by qPCR. n = 9 (C) After transfection with EGFR interference vector, the growth curves of GC were assessed by CCK-8 reagent. n = 8. (D) The proliferation state of GCs was determined by EdU assay after transfection of si-EGFR. n = 3. (E) The apoptosis state of GCs was determined by TUNEL assay after transfection of si-EGFR. n = 3. (F) After transfection with EGFR interference vector, the protein levels of β-tubulin, EGFR, Caspase9, ERK1/2, p-ERK1/2, AKT and p-AKT were assessed by western blot. n=3. Values represent mean ± SEM. * P < 0.05 and ⁎⁎P < 0.01.