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GSH intervention treatment for TiO2 NP exposure. (A) Immunostaining of Orb (green) in control, TiO2 NP- (1.8 mg/mL), TiO2 NP- (1.8 mg/mL) + GSH (0.015 and 0.15 mg/mL)-exposed testes. DNA was stained with Hoechst33342 (blue). (B) The number of Orb-positive spermatid cluster. ***P < 0.001, n.s. represents no statistical difference. Scale bar: 50 μM.
