| instrument |
Shimadzu GC2010 (Kyoto, Japan) |
Thermo Trace GC Ultra
(Thermo Fisher, Bremen, Germany) |
| injector |
Split/splitless (SSL) injector |
Programmable
temperature vaporizer (PTV) |
| column parameters |
TG5MS (Thermo Fisher Scientific;
Waltham, MA, USA) |
| length: 30 m length |
| internal diameter: 0.25 mm |
| stationary phase thickness: 0.25
μm |
| liner |
deactivated packed wool glass SSL inlet liner (Shimadzu, Sydney,
Australia) |
straight deactivated PTV metal liner (Thermo
Fisher Scientific;
Waltham, MA, USA) |
| injection
parameters |
injection
volume: 1 μL |
| temperature: 300 °C |
| split ratio: 1:25 |
| carrier gas: helium |
| flow programming |
mode: flow control |
| carrier gas flow rate: 2 mL/min |
| oven temperature programming |
60 °C (2 min), 10 °C/min
to 300 °C (9 min) |
| total run time: 34 min |
| detectors |
TQ8040 MS (triple quadrupole) |
flame ionization detector (FID), TSQ Quantum XLS MS (triple
quadrupole) |
| FID parameters |
|
temperature: 300 °C |
| airflow: 350 mL/min |
| H2-flow: 35 mL/min |
| makeup (N2) flow: 30 mL/min |
| MSD
parameters |
transfer
line temperature: 250
°C |
| electron
ionization (EI) source
temperature: 200 °C |
| electron energy: 70 eV |
| emission current: 60 μA |
| filament power switch time:
0
min off, 6 min on |
| monitoring ions: |
| 1. Full scan (m/z 40 to 500); scan
time: 0.3 s |
| 2. Multiple reaction monitoring
(MRM, parameters see Figure 1); |
| collision
gas: Argon |
| data acquisition and evaluation
software |
GCMSsolution version 4.52 (Shimadzu) |
Thermo Xcalibur 4.4.16.14 (Thermo Fisher) |
