FIG. 1.

Schematic presentation of the BVDV full-length cDNA clone pOR. The part of the plasmid containing the complete cDNA derived from the BVDV Oregon genome is shown. The upper part indicates the cDNA, with thin bars at the ends representing the nontranslated regions (NTR) and the thick bar in the middle corresponding to the open reading frame coding for the viral proteins, which are also indicated. Solid bars represent the heterologous sequences derived from pA/BVDV, the infectious clone of BVDV CP7 (22). Gray boxes represent the regions coding for the structural proteins, whereas open boxes are indicative of sequences encoding nonstructural proteins. Below the scheme of the genome, blowups of the nontranslated regions and flanking regions containing the T7 RNA polymerase promoter (thin white box) and the SrfI site necessary for runoff transcription are shown. Thin black lines represent plasmid sequences. The XhoI site is located at nt 224 to 229, whereas the AatII site represents nt 12268 to 12273 (numbers refer to BVDV SD1 [8]).