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Growth curve of BVDV Oregon and the virus derived from construct pOR [V(pOR)]. Cells were infected with the viruses at an MOI of 0.02 and harvested by freezing and thawing at the indicated time points. Titers were determined after infection of new cells by counting the number of plaques 72 h postinfection (p.i.) The results are given as log10 PFU per milliliter.
