Skip to main content
. 2000 Jan;74(1):401–410.

FIG. 8.

FIG. 8

CDP/Cut inhibits transient HPV replication in vivo. Cho cells stably expressing E1 and E2 proteins were transfected with pBluescript SK(+) carrying the HPV-16 LCR or with recircularized HPV-31 DNA. Total cellular DNA was prepared after 48 h, digested with DpnI to remove the input bacterially replicated DNA, linearized with EcoRI (HPV-16) or HpaI (HPV-31), respectively, blotted, and hybridized with an LCR fragment of HPV-16 (lanes 1 to 3) or of HPV-31 (lanes 4 to 6). The arrow denotes the position of the linearized replication products, which have molecular sizes of 4 kb in pBluescript SK(+) HPV-16LCR (arrow in the left panel) and 7.9 kb in the linearized HPV-31 genome (arrow in the right panel). Cotransfection of the CDP expression vector pMT2-CDP strongly represses replication for both HPV types (compare lane 1 with lane 3, and lanes 4 with lanes 5 and 6. The expression vector pMT2 without CDP sequences does not interfere with the replication of HPV-16 (lane 2).