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. 2024 Jun 5;25:568. doi: 10.1186/s12864-024-10250-0

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Examples of PCR genotyping analyses. Genotypes of each offspring (IDs indicated by numbers) were verified using three different primer sets (A). (B) A representative example of genotyping of offspring from Project 9 (Table 1). The PCR-positive offspring are indicated in red. (C) Example genotyping of pups obtained by injecting FLPo mRNA into fertilized eggs derived from offspring #56 with extra vector sequence obtained in Project 6 (Table 2). The PCR-positive offspring are indicated in red. N: Negative control. Full sized images and other raw data files relevant to this figure are included in Supplementary Information Figs. 2A-C and 3, and supplementary information files AT1054.TIF, AT1058.TIF and AT1061.TIF and AT0446.TIF