Fig. 6.

Impact of castration on X-linked gene expression in mice. A Distinctive Xist mRNA expression in XX than XY mice [(F (1, 15) = 34); P < 0.0001]. B Kdm5c expression was not impacted by gonadectomy in either XXM or XYM mice [(F (1, 13) = 0.2) P = 0.7]. C Kdm6a expression was significantly higher in castrated XXM mice (t = 3, df = 12; P = 0.01) but not XYM (t = 2, df = 12; P = 0.1). No significant effect of castration as indicated in (D) Ddx3x [(F (1, 16) = 4); P = 0.06] and E Usp9x [(F (1, 16) = 0.3) P = 0.6]. However, F Eif2s3x gene expression was decreased in gonadectomized XXM (t = 4, df = 16; P = 0.02), but not XYM (t = 2, df = 16; P = 0.2) mice. All data sets were computed as mean fold change ± SEM, and 2-Way ANOVA was used to compute column effect, row effect, and their interaction with Sidak’s multiple comparisons. *P < 0.05 and **P < 0.01