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. Author manuscript; available in PMC: 2024 Jun 6.
Published in final edited form as: Nat Biomed Eng. 2022 May 9;6(7):819–829. doi: 10.1038/s41551-022-00888-0

Fig. 5 |. Treatment of melanoma-bearing mice with masked IL-12 does not generate systemic irAEs.

Fig. 5 |

Orthotopic B16F10 melanoma-bearing mice were treated with PBS (n=8), 5 μg (83.3 pmol) IL-12 (n=8) or 250 pmol M-L6-IL12 (n=7) i.v. on days 6 and 9 post tumour inoculation. On day 11, mice were bled for plasma cytokine quantification using LEGENDPlex (a) and spleens were excised for flow cytometric analysis (b). Data are mean ± s.e.m. Experiments were performed twice with similar results. Statistical analyses were performed using ordinary one-way ANOVA with Tukey’s multiple comparison tests.