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. Author manuscript; available in PMC: 2024 Jun 6.
Published in final edited form as: J Am Chem Soc. 2023 Dec 22;146(1):677–694. doi: 10.1021/jacs.3c10406

Figure 5.

Figure 5.

Identification of excited state chemical shift differences extracted from Off-Resonance R1ρ experiments measured on the d(CpG)3. (a) Aromatic 13C-1H HSQC (CH8 of purines and CH6 of pyrimidines) assignments are shown for 8mG4 d(CpG)3 (purple peaks, B-form and Z-form peaks are denoted by subscripts B or Z, respectively) compared to the d(CpG)3 construct at 42°C (folded, blue peaks) and 70°C (melted, red peaks). (b) Chemical shift differences (13C Δω) extracted from Off-Resonance R1ρ experiments measured on the d(CpG)3 construct at 42°C, the difference between the B-form and Z-form peaks in the 13C, 1H HSQC of the 8mG4 d(CpG)3 construct, and the difference between the folded and melted peaks in the 13C, 1H HSQC of the d(CpG)3 construct at 42°C and 70°C. The Z-form chemical shift position for Guanine 4 for the 8mG4 d(CpG)3 construct could not be compared due to the methyl modification.