Table 1.
Duplex constructs’ melting temperatures and NaClO4 Z-form midpoints measured from circular dichroism.
| Construct | TM (°C) | Z-form midpoint (M [NaClO4]) |
|---|---|---|
| d(CpG)3 | 50.24 ± 0.00 | 1.923 ± 0.121 |
| d(5mCpG)3 | 54.04 ± 0.24 | 0.824 ± 0.093 |
| 8mG4 d(CpG)3 | 26.01 ± 0.22 | 0.0044 ± 0.0005 |
| 8mG4 d(CpG)3 (4:1 Zα:RNA) | 59.80 ± 0.27 | |
| d(CpG)6 | 77.77 ± 0.74 | 1.867 ± 0.111 |
| d(CpG)3TG(CpG)2 | 80.78 ± 2.59 | 1.683 ± 0.052 |
| (dCprG)6 | 55.41 ± 0.14 | 1.064 ± 0.157 |
| d(CpG)12 | >100 | 1.770 ± 0.066 |
| r(CpG)3 | 49.22 ± 2.13 | 4.105 ± 0.162 |
| 8mG4 r(CpG)3 | A-form: 49.49 ± 5.38 Z-form: 22.76 ± 0.60 |
0.0164 ± 0.0066 |
| 8mG4 r(CpG)3 (4:1 Zα:RNA) | 64.32 ± 0.32 | |
| r(CpG)6 | 86.36 ± 0.86 | 5.218 ± 0.185 |
| r(CpG)3UG(CpG)2 | 54.02 ± 4.09 | 6.299 ± 0.207 |
| r(CpG)2CIUG(CpG)2 | 38.39 ± 0.46 | 5.013 ± 0.222 |
| r(CpG)12 | >100 | 5.513 ± 0.119 |
| (dCpLG)6 | N/A | N/A |
| r(CpG)3 cUUCGg tetraloop | N/A | >6 |
| r(CpG)3 6mer Ura loop | 77.72 ± 1.82 | >6 |
N/A means that the melting temperature could not be measured over the range measured.