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. Author manuscript; available in PMC: 2024 Jun 6.
Published in final edited form as: J Am Chem Soc. 2023 Dec 22;146(1):677–694. doi: 10.1021/jacs.3c10406

Table 2.

Conformational exchange parameters from Off-Resonance R1ρ relaxation dispersion experiments for tested constructs using a global fitting routine.

Construct Residue/Atom identity Temperature (°C) kex (s−1) pE (%) Δω (ppm)
d(CpG)3 Gua2/C8 42 1630 ± 140 4.0 ± 0.6 1.35 ± 0.12
Cyt3/C6 42 1630 ± 140 4.0 ± 0.6 0.87 ± 0.07
Gua4/C8 42 1630 ± 140 4.0 ± 0.6 1.11 ± 0.09
Cyt5/C6 42 1630 ± 140 4.0 ± 0.6 0.91 ± 0.08
d(5mCpG)3 Cyt3/C6 42 1300 ± 280 2.0 ± 0.5 1.32 ± 0.25
Gua4/C8 42 1300 ± 280 2.0 ± 0.5 1.69 ± 0.22
Cyt5/C6 42 1300 ± 280 1.93 ± 0.06 1.18 ± 0.21
r(CpG)3 Gua2/C8 42 977 ± 53 1.93 ± 0.06 2.17 ± 0.08
Cyt3/C6 42 977 ± 53 1.93 ± 0.06 2.20 ± 0.07
Gua4/C8 42 977 ± 53 1.93 ± 0.06 3.76 ± 0.07
Cyt5/C6 42 977 ± 53 1.93 ± 0.06 2.45 ± 0.06
Gua6/C8 42 977 ± 53 1.93 ± 0.06 2.47 ± 0.06

Only exchange parameters for profiles that could be fit reliably are shown.