Table 3.
B- and Z-form populations in the 8mG4 d(CpG)3 constructs at different temperatures determined from peak volume integration.
| Residue/Atom identity | B-form population (%) at 5°C | B-form population (%) at 15°C | B-form population (%) at 20°C | B-form population (%) at 25°C | B-form population (%) at 30°C | B-form population (%) at 35°C |
|---|---|---|---|---|---|---|
| Cyt1/C1’ | 12.5 | 14.0 | 13.3 | 9.3 | 3.9 | 2.9 |
| Gua2/C1’ | 17.3 | 14.8 | 16.5 | 10.3 | 9.4 | 2.7 |
| Gua2/C8 | 16.9 | 10.7 | 7.9 | 4.9 | 1.3 | 0.51 |
| Gua6/C8 | 11.1 | 9.6 | 8.8 | 8.8 | 0.82 | 0.36 |
| average | 14.4 ± 3.1 | 12.3 ± 2.5 | 11.6 ± 4.0 | 8.3 ± 2.3 | 3.9 ± 3.9 | 1.6 ± 1.4 |