Table 4.
A- and Z-form populations in the 8mG4 r(CpG)3 constructs at different temperatures determined from peak volume integration.
| Residue/Atom identity | B-form population (%) at 5°C | B-form population (%) at 15°C | B-form population (%) at 25°C | B-form population (%) at 35°C | B-form population (%) at 45°C | B-form population (%) at 25°C (100 mM NaClO4) |
|---|---|---|---|---|---|---|
| Cyt1/C5 | 41.3 | 49.6 | 43.7 | 18.4 | 17.1 | 23.3 |
| Cyt1/C6 | 44.1 | 55.8 | 54.9 | 24.5 | 14.9 | 26.4 |
| Gua2/C8 | 40.6 | 40.3 | 50.8 | 39.0 | 26.3 | |
| Cyt3/C5 | 40.9 | 50.1 | 41.3 | 18.5 | 20.2 | |
| Cyt3/C6 | 48.1 | 55.8 | 47.7 | 42.4 | 28.3 | |
| Cyt5/C1’ | 67.7 | 56.72 | 49.7 | 45.9 | 34.2 | |
| Cyt5/C5’ | 48.2 | 51.4 | 50.0 | 15.9 | 27.4 | |
| Cyt5/C5” | 49.2 | 54.2 | 51.2 | 29.7 | 26.4 | |
| Cyt5/C5 | 51.6 | 51.2 | 56.2 | |||
| Cyt5/C6 | 44.5 | 54.7 | 60.0 | 49.5 | ||
| Gua6/C8 | 41.0 | 49.5 | 49.7 | 29.5 | ||
| average | 47.0 ± 7.84 | 51.8 ± 4.6 | 50.5 ± 5.3 | 31.3 ± 12.2 | 16.0 ± 1.5 | 26.6 ± 4.0 |