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Immunoblot analysis of UL25 expression. Affinity-purified pelB-UL25-His fusion polypeptide (lane 1), protein extracts of COS-7 cells transfected with pCG-UL25 (lane 2) or BSR cells infected with PrV (lane 3), purified PrV Kaplan strain virions (7 × 105 PFU; lane 4), and mock-infected BSR cell extracts (lane 5) were subjected to denaturing SDS–12% PAGE and then immunoblotted with polyclonal mouse anti-UL25 antibodies.
