FIG. 3.

Expression kinetics of the UL25 protein. (A) BSR cells were infected with PrV at an MOI of 5, radiolabeled for 1 h starting at the times indicated in hours postinfection (h p.i.), and then chased for 30 min and harvested for immunoprecipitation with the polyclonal mouse antibodies anti-UL25 (upper panel), anti-gC (middle panel), and anti-gB (lower panel). The immunoprecipitated proteins were separated by SDS–10% PAGE. (B) BSR cells were infected at an MOI of 5 in the absence or presence of 300 μg of PAA per ml, and at 6 h postinfection cells were harvested for immunoblot analysis with the anti-gB, anti-gC, and anti-UL25 antibodies as indicated below each panel.