FIG. 5.

Effect of cytoskeletal drugs on the localization of UL25, UL19, and gB in transiently transfected COS-7 cells. At 24 h after mock transfection (D, E, and F) or after transfection with pCG-UL25 (A, B, and C), pCG-UL19 (G, H, and I), and pCG-gB (J, K, and L), cells were incubated for 2 h with OptiMEM alone (A, D, G, H, J, and K) or with OptiMEM containing either cytochalasin D (B and E) or nocodazole (C, F, I, and L). With the exception of cells in panels G and J, which were directly fixed, cells were extracted with 1% NP-40 before fixation and analyzed with anti-UL25 (A to F), anti-capsid (G, H, and I), and anti-gB (J, K, and L) antibodies. Secondary antibodies were donkey anti-rabbit antibody coupled to DTAF and anti-mouse antibody coupled to Texas red. Magnification, ×750.