FIG. 7.

Effect of cytochalasin D and nocodazole on the localization of UL25 in PrV-infected cells. COS-7 cells in absence of drugs (A to C) or treated with cytochalasin D (D to F) or nocodazole (G to I) were infected with PrV at an MOI of 50, and at 10 min (A, B, D, E, G, and H) or 30 min (C, F, and I) postinfection, the cells were extracted with 1% NP-40, fixed, and analyzed by double immunofluorescence with the rabbit anti-tubulin antiserum (A, D, and G) and the mouse anti-UL25 antibody (B, C, E, F, H, and I). Arrows in panels A and D indicate the microtubule-organizing center. Secondary antibodies and visualization were as described in the legend to Fig. 6. The same fields were photographed in panels A and B, D and E, and G and H. Magnification, ×750.