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Copper coordination to βB2-crystallin. Titration of βB2-crystallin 0.3 mM by Cu2+ (0 to 3.5 equiv) as followed by electron paramagnetic resonance (EPR) (A) and circular dichroism (CD) (B). Each spectrum represents the incremental addition of 0.25 (for CD) or 0.5 (for EPR) equiv. (light to dark blue traces). Parallel region of the EPR spectra is shown in the inset of (A).
