FIG. 1.
Colocalization of ICP22 and UL4 protein with UL3 protein. Rabbit skin cells were exposed to HSV-1 recombinant virus R8105 (A to I) or R4660 (J to L) at a ratio of 10 PFU/cell. At 17 h after infection, the cells were fixed with cold ethanol and reacted with the indicated antibodies. Primary antibodies were diluted 1:500 to 1:1,000. Goat anti-rabbit immunoglobulin conjugated to Texas red (Molecular Probes, Eugene, Oreg.) was used to reveal the antigen reacting to the rabbit antisera against UL3, UL4, and ICP22, while anti-mouse immunoglobulin conjugated to FITC (Sigma) was used to visualize the mouse monoclonal antibody CH28-2. The slides were examined under a Zeiss confocal fluorescence microscope, and laser-scanned digitized images of the cells stained with fluorescent antibody were acquired with software provided with the Zeiss confocal microscope. Ab., antibody.