FIGURE 3.

Integrin CD29 mediates HAFSC‐EVs trafficking to cDC2. (a) Heatmap displaying gene expression of ITGB1 and ITGB3 across diverse immune cell populations in mouse splenocytes (GSE108097) and human PBMC (GSE107011). Log‐transformed transcripts per million (log2(TPM+1)) values were used. Colour scale indicates relative expression levels (red for higher, blue for lower). (b) Itgß1 protein expression on immune cells by FACS analysis on total murine splenocytes (n = 4) and human PBMC (n = 7) reported as MFI ± SD. (c, d) CD29 expression by murine and human cDC1 and cDC2 reported as percentage of CD29 positive cells and as MFI ± SD (*p < 0.05 **p < 0.005, unpaired t‐test, two‐tailed). (e, f) Flow cytometry analysis of EVs‐Dil⁺ murine and human cDC2 after treatment with CD29 neutralizing or Isotype antibody (as control). Data are shown as mean ± SD of MFI or percentage of Dil⁺cDC2 (n = 3, *p < 0.05; **p < 0.01, ***p < 0.001 by one‐way ANOVA with Bonferroni's multiple comparison test).