Extended Data Fig. 9. Brain clearance is uniform across the brain.
The concentration of AF488 dye 3 hours after injection into the CPu was measured at an anterior-posterior coordinate 1 mm from the site of injection. a, The concentration of dye was then calculated as a function of radial distance from the peak concentration in both the dorsal and ventral directions. As predicted by equation [8], this results in a Gaussian curve. b, Using these data (predicted by equation [8]) together with equation [2], the percentage clearance can be calculated in the dorsal and ventral directions. Two-way ANOVA shows that there is no significant change in brain clearance across the brain (p = 0.99) for both WAKE animals and those anesthetized by ketamine-xylazine. In contrast, the inhibition of clearance by ketamine-xylazine is highly significant (p < 10−6). For both panels the means are for n = 3 animals and the error envelope shows the SEMs.