Figure 1.

Both 45% and 60% HFD increase body mass compared to CD, but only 60% HFD induces hyperphagia. (A) Nutrient compositions of the 60% CD, 60% HFD, 45% CD, and 45% HFD from Research Diets, Inc. CDs are sucrose matched to their respective HFD, meaning if matched CD and HFD mice eat the same amount of calories, then they will ingest the same amount of sucrose. Macronutrient percentages are given in kcal%. (B) Experimental timeline. (C) Estimated average amount of food consumed per mouse per week (grams) for 60% HFD compared to 60% CD and 45% HFD compared to 45% CD. Measurements represent weekly (datapoint; n = 22) diet's mean ± SD. Two-tailed unpaired t-test statistic with Welch's correction. (D) Estimated average amount of food consumed (grams) per mouse separated per week over the course of the study. Weekly measurements (datapoint) represent the average amount of food consumed per mouse per diet for that week. Each treatment group filled only one cage, so average amount of food eaten per cage per week was divided by 5 for estimated average eaten per mouse per week, leaving all 5 mice in the condition with the same amount of food eaten. No statistical analysis was performed. (E) Average mouse body weight (grams) per diet over the course of the experiment. Measurements represent weekly (datapoint; n = 5 per condition) diet mean ± SD. Multiple unpaired t-tests statistic with Welch's correction. (F) Total amount of weight gained (grams) per mouse after 22 weeks on diet. Measurement (datapoint; n = 5 per condition) represents each mouse (bar = mean ± SD). Two-tailed unpaired t-test statistic. (G) Percent body weight gained by the end of the experiment (22 weeks on diet). Measurement (datapoint; n = 5 per condition) represents each mouse (bar = mean ± SD). Two-tailed unpaired t-test statistic. (H) Images of mouse body size after 22 weeks on diet. *P < .05; **P < .01; ***P < .001; ****P < .0001.

Abbreviations: CD, control diet; HFD, high-fat diet.