Fig. 1.

The effects of forsythiaside A on the inflammation of lung and colon in LPS-induced ALI mice. (A) Schematic diagram of DEX and forsythiaside A treatment in LPS-induced ALI mice; (B) Lung weight (mg, n = 8, per group); (C) Lung index (mg/g, n = 8, per group); (D) HE-staining representative images of lung tissues (20 × and 200 × magnifications); (E) Inflammation scores of lung tissues stained by HE (n = 6, per group); (F) WB bands and relative protein expressions of inflammatory indicators (iNOS, TNF-α, IL-1β and IL-6) in lung tissues (n = 6, per group); (G) HE-staining representative images of colonic tissues (100 × and 200 × magnifications); (H) WB bands and relative protein expressions of inflammatory indicators in colonic tissues (n = 6, per group); (I) IHC-staining images of macrophage-related protein F4/80 in lung tissues (200 × magnifications); (J) IHC-staining images of F4/80 in colon tissues (200 × magnifications). DEX referred to 5 mg/kg of dexamethasone, and LFA, MFA and HFA referred to 20, 40 and 80 mg/kg of forsythiaside A, respectively. Data were expressed as mean ± SD. *P < 0.05, **P < 0.01 versus MOD; ^#P < 0.05, ^##P < 0.01 versus CON.