The effects of forsythiaside A on the inflammation and TLR4/MAPK/NF-κB signal pathway in LPS-induced RAW264.7 cells. (A) Schematic diagram of forsythiaside A treatment in LPS-induced RAW264.7 cells; (B) Representative morphological images of LPS (1 μg/mL)-induced RAW264.7 cells with the treatment of forsythiaside A for 24 h; (C) and (D) WB bands and relative protein expressions of inflammatory indicators (iNOS, TNF-α, IL-1β and IL-6) in the cells; (E) Levels of TNF-α, IL-1β and IL-6 in the supernatants; (F) WB bands and relative protein expressions of TLR4/MAPK/NF-κB-related proteins in RAW264.7 cells with the induction of LPS and the treatment of forsythiaside A for 24 h. LFA, MFA and HFA referred to the Low-, Medium- and High-doses (50, 100 and 200 μM) of forsythiaside A, respectively. Data were expressed as mean ± SD (n = 3). *P < 0.05, **P < 0.01 versus LPS; #P < 0.05, ##P < 0.01 versus CON.