Fig. 1.

Ipsc-derived airway organoids (ao) exhibit ace2 expression and vulnerable to sars-cov-2 pseudovirus infection. (A) Schematic illustration of the differentiation process of iPSCs to AO. (B) Brightfield image of the iPSC-derived AO at different stages of differentiation spanning ≧ 30 days. Scale bar = 200 µm. (C) Immunofluorescence staining of AO showed marker expression of basal cell (KRT5), secretory cell (SCGB3A2 and CFTR), and ciliated cell (Act-α-TUB). Nuclei and cellular actin filaments were counterstained with DAPI (blue) and ACTIN (green), respectively. Scale bar = 20 µm. (D) Cluster map showing the assigned identity for each airway component (Multiciliated, Secretory, Basal, AT1, and AT2 cells). (E) t-SNE projection showing major airway marker CDK1, CEACAM6, KRT17, OBSL1, TTYH1, and ACE2 expressions in the AO. (F) Immunoblotting analysis showed high ACE2 protein expression in iPSC-derived AO. (G) iPSC-derived AO expressed GFP signals after 48 h infection with GFP-conjugated SARS-CoV-2 pseudovirus. Scale bar = 100 µm. (H) Quantitative real-time PCR analysis showed increased mRNA expression level of GFP; n = 3. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)