REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti-Lgi1/EPT antibody	Abcam	Cat# ab30868; RRID: AB_776017
Mouse anti-GRIA1 antibody	UC Davis/NIH NeuroMab Facility	Cat# 75-327; RRID: AB_2877405
Goat Anti-Rabbit IgG (H + L)-HRP Conjugate	BioRad	Cat# 1706515; RRID: AB_11125142
Goat Anti-Mouse IgG (H + L)-HRP Conjugate	BioRad	Cat# 1706516; RRID: AB_11125547
V5 Tag Monoclonal Antibody	Invitrogen	Cat# R960-25; RRID: AB_2556564
Beta Actin Polyclonal Antibody	Thermo Fisher Scientific	Cat# PA5-85271; RRID: AB_2792414
Bacterial and virus strains
FSW-HRP-V5-LRRTM1	PF iVector-ICM	Addgene #82536
FSW-HRP-V5-LRRTM2	PF iVector-ICM	Addgene #82537
Biological samples
Human LGI1 antibodies	Jena University Hospital	licence # 2019-1415 -Material
Chemicals, peptides, and recombinant proteins
Bafilomycin A1	Cayman Chemical Company	Item #11038
EGTA-AM	Thermo Fisher Scientific	E1219
Tetrodotoxin citrate (TTX)	Tocris	Cat #1069
Biotin-XX Tyramide Reagent (BxxP)	ApexBio Technology	Cat #A8012
Tetanus toxin from Clostridium tetani	Sigma	T3194
Recombinant Enhanced GFP protein (His tag)	Abcam	ab134853
Papain	Worthington Biochemical	LK003178
DNase I	Roche	10104159001
Poly-D-lysine	Sigma	P2636
MEM, no glutamine, no phenol red	Thermo Fisher Scientific	51200038
N21-MAX Media Supplement (50X)	Bio-Techne	AR008
Transferrin	Sigma	616420
Glutamax	Thermo Fisher Scientific	35050038
Insulin	Sigma	I6634
Cytosine β-d-arabinofuranoside	Sigma	C1768
Neurobasal™ Medium	Thermo Fisher Scientific	21103049
BrainPhys™ Neuronal Medium and SM1 Kit	STEMCELL Technologies	05792
5′-fluoro-2′-deoxyuridine (FUDR)	Fisher Scientific	10144760
6-cyano-7-nitroquinoxaline-2,3-dione (CNQX)	Tocris	1045
D-(−)-2-Amino-5-phosphonopentanoic acid (AP5)	Tocris	0106
MES (2-(N-morpholino)ethanesulfonic acid)	Sigma	M3671
ProLong™ Glass Antifade Mountant	Thermo Fisher Scientific	P36982
Lipofectamine™ 2000 Transfection Reagent	Thermo Fisher Scientific	11668019
Chloroquine diphosphate salt, 98%	Thermo Fisher Scientific	455245000
Pierce™ Streptavidin Magnetic Beads	Thermo Fisher Scientific	88817
Biotin	Sigma	B4501
DL-Dithiothreitol (DTT)	Sigma	D9779
Clarity™ Western ECL Substrate	BioRad	1705060
Clarity Max Western ECL Substrate	BioRad	1705062
Critical commercial assays
Q5® Site-Directed Mutagenesis Kit	New England Biolabs	E0552
Pierce™ High Sensitivity Streptavidin-HRP	Thermo Fisher Scientific	21130
NEBuilder® HiFi DNA Gibson Assembly Master Mix	New England Biolabs	E2621
p24 ZeptoMetrix Kit	Sigma	0801111
Experimental models: Cell lines
HEK293T cells	ATCC	CRL-11268
Experimental models: Organisms/strains
Sprague-Dawley Rat	Charles River	Strain: Crl:CD(SD)
Oligonucleotides
See Table S2 for oligonucleotides used for site-directed mutagenesis	This paper	N/A
Recombinant DNA
CamKII-LGI1-pHluorin	This paper	Addgene Plasmid #185537
CamKII-LGI1-S473L-pHluorin	This paper	Addgene Plasmid #185540
CamKII-LGI1-Y433A-pHluorin	This paper	Addgene Plasmid #185541
CamKII-LGI1-R474Q-pHluorin	This paper	Addgene Plasmid #185542
CamKII-LGI1-T380A-pHluorin	This paper	Addgene Plasmid #185543
CamKII-LGI1-E383A-pHluorin	This paper	Addgene Plasmid #185544
CamKII-LGI1-C200R-pHluorin	This paper	Addgene Plasmid #185545
CamKII-LGI1 (no tags)	This paper	N/A
vGlut-pH	Gift from Timothy A. Ryan34	N/A
vGlut-mOrange-2	Hoppa et al., 201280	N/A
iGluSnFR3	Aggarwal et al.58	Addgene Plasmid #178330
CMV-mRuby-synapsin	Gift from Timothy A. Ryan	Addgene Plasmid #187896
pKanCMV-mRuby3-18aa-actin	Bajar et al.86	Addgene plasmid #74255
pLKO-synapsin-BFP	Gift from Timothy A. Ryan	Addgene Plasmid #191567
pLKO-empty-mTagBFP	Gift from Timothy A. Ryan	Addgene Plasmid #191566
CamKII-ADAM23-pHluorin	This paper	Addgene Plasmid #185539
pGP-AAV-syn-jGCaMP8f-WPRE	Zhang et al.55	Addgene Plasmid #162376
CamKII-LGI1-pHmScarlet	This paper	Addgene Plasmid #185538
CamKII-LGI1-R474Q-pHmScarlet	This paper	N/A
CamKII-LGI1-T380A-pHmScarlet	This paper	N/A
CamKII-LGI1-E383A-pHmScarlet	This paper	N/A
pCAGGS-SNAP25ΔC-IRES-mCherry	Ibata et al., 201943	N/A
pCAGGS-SNAP29ΔC-IRES-mCherry	Ibata et al., 201943	N/A
pCAGGS-Stx1AΔC-IRES-mCherry	Ibata et al., 201943	N/A
pCAGGS-Stx4ΔC-IRES-mCherry	Ibata et al., 201943	N/A
CMV-IgK-pHluorin-TM-mRuby	This paper	Addgene Plasmid #185546
ER-pHluorin	de Juan-Sanz et al., 201736	N/A
FSW-HRP-V5-LRRTM2	Loh et al.52	Addgene Plasmid #82537
FSW-HRP-V5-LRRTM1	Loh et al.52	Addgene Plasmid #82536
pAAV-CaMKII-Archon1-EGFP	Piatkevich et al.60	Addgene Plasmid #108417
hSyn-NPY-pHluorin	Gift from Ruud F Toonen Persoon et al. 87	N/A
VAMP2-pHmScarlet	Gift from Pingyong Xu Liu et al.54	Addgene plasmid #166890
CMV-mRuby-synapsin	Gift from Timothy A. Ryan	Addgene Plasmid #187896
pLKO-synapsin-BFP	Gift from Timothy A. Ryan	Addgene Plasmid #191567
pLKO-empty-mTagBFP	Gift from Timothy A. Ryan	Addgene Plasmid #191566
CamKII vector	Gift from Edward Boyden Chow et al. 88	Addgene Plasmid #22217
Vesicular stomatitis virus G glycoprotein	Gift from Irving WeissmanHaselmann et al. 89	Addgene Plasmid #14888
Software and algorithms
ImageJ	NIH Schneider et al. 90	https://imagej.nih.gov/ij/
GraphPad Prism 8	GraphPad Software	https://www.graphpad.com/scientific-software/prism/
R	R-project	https://www.r-project.org
RStudio	RStudio	https://www.rstudio.com
Biorender	Biorender	https://www.biorender.com/
Other
ChemiDocTM Touch Imaging System	BioRad	1708374
Custom-built laser illuminated epifluorescence Microscope Axio Observer 3	ZEISS	N/A
iXon Ultra camera	Andor - Oxford Instruments	#DU-897U-CSO-#BV
Oasis™ UC160 Cooling System	Solid State Cooling Systems	UC160
Imaging chamber	Warner Instruments	RC-21BRFS
Feedback loop temperature controller	Warner Instruments	TC-344C