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| Target gene | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|
| Nfatc1 | CAGTGTGACCGAAGATACCTGG | TCGAGACTTGATAGGGACCCC |
| Osterix | CGCTTTGTGCCTTTGAAAT | CCGTCAACGACGTTATGC |
| Runx2 | AGGGACTATGGCGTCAAACA | GGCTCACGTCGCTCATCTT |
| Gapdh | TGGGTGTGAACCATGAGAAG | GCTAAGCAGTTGGTGGTGC |
Nfatc1, the nuclear factor of activator T-cells, cytoplasm 1; Runx2, Runt-related transcription factor 2; Gapdh, glyceraldehyde 3-phosphate dehydrogenase.
