Table 3. Effects of Ka on the antioxidant enzyme activities and H2O2 level of the blood and liver in C57BL/6J mice fed an HFD.
| Variables | ND | HFD | HFD + Ka | |
|---|---|---|---|---|
| Blood | ||||
| SOD (unit/mg Hb) | 1.65 ± 0.02 | 1.71 ± 0.05 | 1.75 ± 0.04 | |
| CAT (µmol/min/mg Hb) | 0.228 ± 0.003 | 0.195 ± 0.005** | 0.216 ± 0.002## | |
| GSH-Px (nmol/min/mg Hb) | 9.36 ± 0.43 | 8.14 ± 0.25* | 8.41 ± 0.22 | |
| Erythrocyte H2O2 (mM/mg Hb) | 0.116 ± 0.014 | 0.148 ± 0.006* | 0.130 ± 0.006# | |
| Plasma H2O2 (µM) | 60.53 ± 8.45 | 61.66 ± 9.76 | 47.24 ± 5.17 | |
| Liver | ||||
| SOD (unit/mg protein) | 93.96 ± 4.71 | 133.15 ± 3.39*** | 106.71 ± 3.22### | |
| CAT (µmol/min/mg protein) | 0.72 ± 0.03 | 1.02 ± 0.06* | 1.30 ± 0.09# | |
| GSH-Px (nmol/min/mg protein) | 10.00 ± 0.18 | 9.52 ± 0.14 | 9.87 ± 0.26 | |
| Cytosolic H2O2 (µM/mg protein) | 7.42 ± 0.50 | 9.84 ± 0.63* | 8.46 ± 0.43 | |
| Mitochondrial H2O2 (µM/mg protein) | 13.32 ± 0.37 | 21.69 ± 1.06*** | 15.15 ± 0.94## | |
Data are expressed as the mean ±SE of the mean.
ND, normal diet (AIN-76); HFD + Ka, high-fat diet + 0.02% kaempferol; SOD, superoxide dismutase; Hb, hemoglobin; CAT, catalase; GSH-Px, glutathione peroxidase.
Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001 vs. ND; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. HFD.