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. 2024 Jun 6;15:4842. doi: 10.1038/s41467-024-49090-7

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — For each reaction shown, the net fluxes are normalized to 100 units of CO₂ taken up. All flux values and confidence intervals are given in Supplementary Data 4. Estimated net CO₂ uptake rates for TG1, TG1-MYB99, and TG2 are 5.96, 6.99, and 9.59 mmol gBiomass^-1 h^-1 respectively (derived from flux models, using n = 3 biological replicated experiments). Ribulose 1,5-bisphosphate (RuBP), 3-phosphoglycerate (3PGA), Triose phosphate (TP), Fructose 1,6-bisphosphate (FBP), Fructose 6-phosphate (F6P), Glucose 6-phosphate (G6P), Glucose 1-phosphate (G1P), Ribulose 5-phosphate (Ru5P), Erythrose 4-phosphate (E4P), Ribose 5-phosphate (R5P), Xylulose 5-phosphate (X5P), Sedoheptulose-7-phosphate (S7P), Sedoheptulose-1,7-bisphosphate (SBP), Phosphoenolpyruvate (PEP), Acetyl-CoA (ACA), Pyruvate (PYR), Glycine (Gly), Serine (SER), 2-phosphoglycolate (2PG), Glycolic acid (GA), Citrate (CIT), Alpha-ketoglutarate (AKG), Succinic semialdehyde (SSA), Succinate (SUC), Fumarate (FUM), Malate (MAL), Oxaloacetate (OAA). Black arrows represent intracellular flux while red arrows represent fluxes to/from biomass sink. The width of the black arrows is indicative of the magnitude of the flux. The red boxes highlight reactions catalyzed by phosphoglucomutase (PGM) and triose phosphate transporter (TPT3) which are implicated in the differing biomass composition phenotypes.