TABLE 1.

Infection of T cells by HIV IC bound to tonsil B cells^a

Time after HIV IC bound (h)	Level of virus replication (pg of p24/ml) in:
	B cell-bound HIV IC	Protease-treated cells	Supernatant
0	16,787 ± 1,487	106 ± 1	NAb
24	17,616 ± 1,899	506 ± 1	352 ± 50
48	19,007 ± 1,791	537 ± 5	346 ± 69
72	20,106 ± 1,576	150 ± 30	979 ± 223

^aTonsil B cells were incubated with HIV IC made with PI 1 plus autologous serum and complement. Autologous serum alone at a 1:30 dilution did not significantly neutralize PI 1 (data not shown). Cells were washed and cultured for 72 h, and at 0, 24, 48, and 72 h of culture, cells and culture supernatants were harvested. Cells were treated with or without proteinase K as described in the Fig. 3 legend. Cells and supernatants were cultured with 2 × 10⁶ PHA-stimulated PBMC per well in 24-well plates, and HIV replication in PBMC cultures was assessed by measuring the p24 core antigen in the supernatants after 12 additional days of culture. The mean level of p24 detected in cultures of B cells incubated with HIV IC in the absence of PBMC-derived T cells on days 7 and 12 were 62 and 77 pg/ml, respectively. The means ± standard errors of the means of results from triplicate cultures are shown. Results shown are representative of two experiments. 

^bNA, not applicable.