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. 2024 Jun 6;223(9):e202405025. doi: 10.1083/jcb.202405025

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© 2024 Dresselhaus et al.

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Figure 1. — Tsg101 is required for the release of EV cargoes from presynaptic terminals. (A–D) Representative confocal images from larvae expressing UAS-Tsg101-RNAi (Tsg101^KD) or a control RNAi either pan-neuronally (C380-GAL4) or in motor neurons (Vglut-GAL4) together with the following EV cargoes: (A) Syt4-GFP expressed from its endogenous locus, (B) UAS-driven Evi-GFP, (C) UAS-driven APP-GFP, and (D) endogenous Neuroglian (Nrg, neuronal isoform Nrg180) detected by antibody. (E–H) Quantification of EV cargo puncta intensity. All images show MaxIPs of muscle 6/7 segments A2 or A3. Scale bars are 5 µm. (A–D) Blue outlines represent the neuronal membrane as marked from an HRP mask; the yellow line in A shows a 3.3 µm dilation of the HRP mask, representing the postsynaptic region. Arrows show examples of postsynaptic EVs. Data are represented as mean ± SEM; n represents NMJs. All intensity measurements are normalized to their respective controls. *P < 0.05, ***P < 0.001. See Tables S1 and S3 for detailed genotypes, sample sizes, and statistical analyses.