TABLE 1.
Primers used for amplification of marked genes of O. tsutsugamushi.
| Gene | Primer name | Sequences (5′→3′) |
Anneal temperature | Length of coding sequence | References |
| 56-kDa | P34-F1 | TCAAGCTTATTGCTAGTGCAATGTCTGC | 55°C | _ | Furuya et al., 1993 |
| P55-R1 | AGGGATCCCTGCTGCTGTGCTTGCTGCG | ||||
| P10-F2 | GATCAAGCTTCCTCAGCCTACTATAATGCC | 55°C | 485 bp | ||
| P11-R2 | CTAGGGATCCCGACAGATGCACTATTAGGC | ||||
| groEL | Gro-F1 | AAGAAGGA/CGTGATAAC | 50°C | _ | Chen et al., 2000 |
| Gro-R1 | ACTTCA/CGTAGCACC | ||||
| Gro-F2 | ATATATCACAGTACTTTGCAAC | 50°C | 365 bp | ||
| Gro-R2 | GTTCCTAACTTAGATGTATCAT |