FIG. 3.

Role of TRAIL in the cytotoxic activity of MV-infected DCs and monocytes. DCs and monocytes were either mock treated (Mock-DCs and Mock-Mono, respectively) or MV infected (MV-DCs and MV-Mono, respectively), then placed in culture for 12 h. Harvested monocytes (A) and DCs (B) were then cultured with ⁵¹Cr-labeled MDA-231 target cells at the indicated effector-to-target cell (E/T) ratios. TRAIL-R2:Fc or TNF-R1:Fc chimeras (20 μg/ml) were added or not added to the assay to inhibit TRAIL or TNF-α-induced cell death. ⁵¹Cr release was measured 8 h later. Results are means of data from three experiments; in all cases, standard deviations were below 8%.