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. 2024 Jun 6;7(8):e202302503. doi: 10.26508/lsa.202302503

Figure 2. STAT3 deletion in myofibers has no impact on the autophagic process.

Figure 2.

(A) Schematic representation of the ex vivo experiment in myofibers isolated from young STAT3f/f (WT) or MCK-Cre;STAT3f/f mice (mKO), and cultured in growth medium (GM). To evaluate the autophagic flux, CLQ was administered 4 h before harvesting. (B) STAT3 protein expression in isolated myofibers from WT and mKO mice. The plot represents the STAT3/GAPDH ratio (n = 3; values represent the mean ± s.d., ***P < 0.001 by a t test). (C) LC3 protein expression in isolated myofibers from WT and mKO mice. The plot represents the relative change in the LC3II/GAPDH ratio between CLQ-treated and not treated samples (ΔLC3II/GAPDH) in WT and mKO mice (n = 4; values represent the mean ± s.d., not significant [ns] by a t test).